Journal: Journal of cellular biochemistry

Article Title: The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway.

doi: 10.1002/jcb.26212

Figure Lengend Snippet: Fig. 1. Verification of the isolation results of human DPSCs. Note: A, primary culture of DPSCs under a microscope (40); B, monoclonal colony formation of DPSCs under a microscope (40); C, immunochemistry image showing CD34, CD45, and CD146 expression; DPSCs, dental pulp stem cells.

Article Snippet: The cell suspension (100ml) was added to a Eppendorf tube (3.0 109/tube), and then incubated with rat-anti-human monoclonal antibodies CD105-PE (bs-0579RPE, Bioss Biotechnology Co., Ltd, Beijing, China), CD29-PE (bs0486R-PE, Bioss Biotechnology Co., Ltd), CD34-PE (bs-0646R-PE, Bioss Biotechnology Co., Ltd), CD45-PE (bs-0522R-PE, Bioss Biotechnology Co., Ltd), CD90-PE (bs-0778R-PE, Bioss Biotechnology Co., Ltd), CD146-PE (bs-1618R-PE, Bioss Biotechnology Co., Ltd) and rat-anti-human STRO-1 (ab214086, Abcam Inc., Cambridge, MA) at 4°C for 30min, followed by incubation for 15min in the dark, washing with buffer solution, centrifugation at 1000 rpm for 5min, suspension and finally fixation with buffer solution containing 1% paraformaldehyde.

Techniques: Isolation, Microscopy, Expressing

Journal: Bioengineering

Article Title: An Approach towards a GMP Compliant In-Vitro Expansion of Human Adipose Stem Cells for Autologous Therapies

doi: 10.3390/bioengineering7030077

Figure Lengend Snippet: Overview of primer sequences used for RT-qPCR measurements.

Article Snippet: The flow cytometric measurements contained different mixtures of the following antibodies: CD26-FITC, CD73-FITC, CD90-APC, CD105-PE (BioLegend, San Diego, CA, USA), CD36-APC, CD146-PE (Miltenyi Biotec, Bergisch Gladbach, Germany), CD55-BV421 (Becton Dickinson, Franklin Lakes, NJ, USA) and CD54-PE (Thermo Fisher Scientific, Waltham, USA).

Techniques:

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: CD146 , REA773 , 50 , 130-111-322 , PE , Miltenyi Biotec.

Techniques: Imaging

Journal: Cell Transplantation

Article Title: Macromolecular Crowding Supports the Generation of Basal Membrane-Rich Pericyte-Based Cell Sheets Useful for Cell Therapy of Diabetic Wounds

doi: 10.1177/09636897241309698

Figure Lengend Snippet: Antibodies Used for Immunofluorescence and FACS Characterization of Pericytes.

Article Snippet: CD146 , PE-Vio615 , 1:50 , Miltenyi , 130-125-731.

Techniques: Immunofluorescence, Conjugation Assay

Journal: Cell Transplantation

Article Title: Macromolecular Crowding Supports the Generation of Basal Membrane-Rich Pericyte-Based Cell Sheets Useful for Cell Therapy of Diabetic Wounds

doi: 10.1177/09636897241309698

Figure Lengend Snippet: Pericytes characterization by FACS and immunofluorescence. Pericytes isolated from human skeletal muscle biopsies by CD146 + magnetic beads sorting express typical pericyte/mesenchymal markers by immunofluorescence (A) and FACS (B) characterization; (C) comparison of pericyte markers expression between control and diabetic patients. Ns: nonstatistically significant, * P < 0.05, ** P < 0.01, *** P < 0.001, N = 10 controls, N = 9 diabetic patients.

Article Snippet: CD146 , PE-Vio615 , 1:50 , Miltenyi , 130-125-731.

Techniques: Immunofluorescence, Isolation, Magnetic Beads, Comparison, Expressing, Control